Application

Research Sub CategoryNuclear Receptors

Anti-KSRP Antibody, clone Ab5, Cat. No. MABE987, is a highly specific mouse Monoclonal antibody, that targets KSRP and has been tested in Western Blotting and Immunocytochemistry.

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: A representative lot detected KSRP in HeLa and mouse neuroblastoma N1E-115 cells (Hall, M.P., et al. (2004). Mol Biol Cell. 15(2):774-786).
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected KSRP in 10 µg of HeLa cell lysate.
Western Blotting Analysis: A representative lot detected KSRP in HeLa and mouse neuroblastoma N1E-115 cells (Hall, M.P., et al. (2004). Mol Biol Cell;15(2):774-86).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Far upstream element-binding protein 2 (UniProt Q92945; also known as FUSE-binding protein 2, KH type-splicing regulatory protein, KSRP, p75) is encoded by the KHSRP (also known as FBP2, FUBP2) gene (Gene ID 8570) in human. The KH-type splicing regulatory protein (KSRP) is an important regulator of several unstable mRNAs coding for pro-inflammatory mediators including TNF-α, IL-8, and iNOS. By binding to AU-rich elements (AREs) in the 3′-untranslated region (3′-UTR), KSRP recruits enzymes involved in the 5′- and 3′-mRNA decay. KSRP interacts with nucleic acids through four hnRNP KH domains and negatively regulates gene expression by promoting decay of target mRNAs. KSRP is also known to associate with Drosha and Dicer complexes and promote the maturation of microRNAs (miRNAs).

Immunogen

Recombinant protein corresponding to human KSRP.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in C2C12 mouse myoblast lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected KSRP in 10 µg of C2C12 mouse myoblast lysate.

Specificity

Two KSRP immunoreactive bands are often observed using HeLa cell extracts, while a single band is seen using other cell extracts (e.g. C2C12 and N1E-115). The nature of the difference between the two bands is not yet known (PMID 14657238 & 9136930).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~80 kDa observed. Uncharacterized band(s) may appear in some lysates.